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| Registros recuperados: 11 | |
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| Homrich,Milena Schenkel; Passaglia,Luciane Maria Pereira; Pereira,Jorge Fernando; Bertagnolli,Paulo Fernando; Salvadori,José Roberto; Nicolau,Marcio; Kaltchuk-Santos,Eliane; Alves,Laura Bianchessi; Bodanese-Zanettini,Maria Helena. |
| The objective of this work was to analyze the agronomic performance and chromosomal stability of transgenic homozygous progenies of soybean [Glycine max (L.) Merrill.], and to confirm the resistance of these plants against Anticarsia gemmatalis. Eleven progenies expressing cry1Ac, hpt and gusA genes were evaluated for agronomic characteristics in relation to the nontransformed parent IAS 5 cultivar. Cytogenetical analysis was carried out on transgenic and nontransgenic plants. Two out of the 11 transgenic progenies were also evaluated, in vitro and in vivo, for resistance to A. gemmatalis. Two negative controls were used in resistance bioassays: a transgenic homozygous line, containing only the gusA reporter gene, and nontransgenic 'IAS 5' plants. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anticarsia gemmatalis; Glycine max; Plant resistance to insects; Gene transfer. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000700003 |
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| Soares,René Arderius; Passaglia,Luciane Maria Pereira. |
| Bradyrhizobium elkanii is successfully used in the formulation of commercial inoculants and, together with B. japonicum, it fully supplies the plant nitrogen demands. Despite the similarity between B. japonicum and B. elkanii species, several works demonstrated genetic and physiological differences between them. In this work Representational Difference Analysis (RDA) was used for genomic comparison between B. elkanii SEMIA 587, a crop inoculant strain, and B. japonicum USDA 110, a reference strain. Two hundred sequences were obtained. From these, 46 sequences belonged exclusively to the genome of B. elkanii strain, and 154 showed similarity to sequences from B. japonicum genome. From the 46 sequences with no similarity to sequences from B. japonicum, 39... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bradyrhizobium; Genome comparison; Nitrogen fixation; Representational difference analysis. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400036 |
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| Giongo,Adriana; Beneduzi,Anelise; Gano,Kelsey; Vargas,Luciano Kayser; Utz,Laura; Passaglia,Luciane Maria Pereira. |
| Microorganisms that live inside and around a plant can supply it with essential substances, such as phytohormones and essential nutrients. The present investigation aimed to isolate and characterize the phyllosphere, the endophytic, and the water tank bacteria associated with Vriesea gigantea and Tillandsia aeranthos. The bacteria were tested for siderophore and indole-3-acetic acid (IAA) production, phosphate solubilization, and presence of the nif H gene. Genetic diversity of the bacterial isolates was evaluated by rep-PCR. Sixty-eight bacterial strains were isolated from 3 different microhabitats of V. gigantea and from 2 microhabitats of T. aeranthos bromeliad plants. Gram-positive, spore-forming bacilli comprised most bacterial isolates. All isolates... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bromeliads; PGPB; Diazotrophic bacteria; Water tank; Siderophore; IAA. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032013000300080 |
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| Roesch,Luiz Fernando Wurdig; Passaglia,Luciane Maria Pereira; Bento,Fátima Menezes; Triplett,Eric W.; Camargo,Flávio Anastácio Oliveira. |
| Bactérias diazotróficas endofíticas são capazes de promover o crescimento do milho por meio da fixação biológica do nitrogênio (FBN) ou pela produção de fitormônios. Neste estudo, objetivou-se caracterizar a diversidade de bactérias diazotróficas endofíticas associadas a plantas de milho em diferentes locais do Rio Grande do Sul, que apresentavam variações de clima e solo. Para isso, foi usado um método baseado na amplificação do gene nifH grupo I, na análise de fragmentos de restrição (PCR-RFLP) e no seqüenciamento dos genes amplificados. Foram calculados os índices de Shannon-Weaver e Equitabilidade para estimar a diversidade dos diazotróficos, bem como a diversidade de nucleotídeos e divergência entre seqüências, para estimar a diversidade genética das... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene nifH; PCR-RFLP; Seqüenciamento; Comunidade endofítica. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832007000600015 |
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| Bücker Neto,Lauro; Oliveira,Rafael Rodrigues de; Wiebke-Strohm,Beatriz; Bencke,Marta; Weber,Ricardo Luís Mayer; Cabreira,Caroline; Abdelnoor,Ricardo Vilela; Marcelino,Francismar Correa; Zanettini,Maria Helena Bodanese; Passaglia,Luciane Maria Pereira. |
| Soybean [Glycine max (L.) Merril], one of the most important crop species in the world, is very susceptible to abiotic and biotic stress. Soybean plants have developed a variety of molecular mechanisms that help them survive stressful conditions. Hybrid proline-rich proteins (HyPRPs) constitute a family of cell-wall proteins with a variable N-terminal domain and conserved C-terminal domain that is phylogenetically related to non-specific lipid transfer proteins. Members of the HyPRP family are involved in basic cellular processes and their expression and activity are modulated by environmental factors. In this study, microarray analysis and real time RT-qPCR were used to identify putative HyPRP genes in the soybean genome and to assess their expression in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fungal disease; HyPRP genes; Glycine max; Real time RT-qPCR. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200012 |
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| Giongo,Adriana; Beneduzi,Anelise; Ambrosini,Adriana; Vargas,Luciano Kayser; Stroschein,Marcos Roberto; Eltz,Flávio Luiz; Bodanese-Zanettini,Maria Helena; Passaglia,Luciane Maria Pereira. |
| Two bacterial strains that amplified part of the nifH gene, RP1p and RP2p, belonging to the genus Enterobacter and Serratia, were isolated from the rhizoplane of Lupinus albescens. These bacteria are Gram-negative, rod-shaped, motile, facultative anaerobic, and fast-growing; the colonies reach diameters of 3-4 mm within 24 h of incubation at 28 ºC. The bacteria were also able to grow at temperatures as high as 40 ºC, in the presence of high (2-3 % w/v) NaCl concentrations and pH 4 -10. Strain RP1p was able to utilize 10 of 14 C sources, while RP2p utilized nine. The isolates produced siderophores and indolic compounds, but none of them was able to solubilize phosphate. Inoculation of L. albescens with RP1p and RP2p strains resulted in a significant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Indolic compounds; Siderophores; Inoculation experiment. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000200009 |
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| Homrich,Milena Schenkel; Passaglia,Luciane Maria Pereira; Pereira,Jorge Fernando; Bertagnolli,Paulo Fernando; Pasquali,Giancarlo; Zaidi,Mohsin Abbas; Altosaar,Illimar; Bodanese-Zanettini,Maria Helena. |
| Somatic embryos of the commercial soybean (Glycine max) cultivar IAS5 were co-transformed using particle bombardment with a synthetic form of the Bacillus thuringiensis delta-endotoxin crystal protein gene cry1Ac, the beta-glucuronidase reporter gene gusA and the hygromycin resistance gene hpt. Hygromycin-resistant tissues were proliferated individually to give rise to nine sets of clones corresponding to independent transformation events. The co-bombardment resulted in a co-transformation efficiency of 44%. Many histodifferentiated embryos and 30 well-developed plants were obtained. Twenty of these plants flowered and fourteen set seeds. The integration and expression of the cry1Ac, gusA and hpt transgenes into the genomes of a sample of transformed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anticarsia gemmatalis; Cry1Ac gene; IAS5 cultivar; Insect resistance; Transgenic soybean. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300020 |
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| Lisboa,Bruno Brito; Bayer,Cimélio; Passaglia,Luciane Maria Pereira; Camargo,Flávio Anastácio de Oliveira; Beneduzi,Anelise; Ambrosini,Adriana; Vargas,Luciano Kayser. |
| Soil management, in terms of tillage and cropping systems, strongly influences the biological properties of soil involved in the suppression of plant diseases. Fungistasis mediated by soil microbiota is an important component of disease-suppressive soils. We evaluated the influence of different management systems on fungistasis against Fusarium graminearum, the relationship of fungistasis to the bacterial profile of the soil, and the possible mechanisms involved in this process. Samples were taken from a long-term experiment set up in a Paleudult soil under conventional tillage or no-tillage management and three cropping systems: black oat (Avena strigose L.) + vetch (Vicia sativa L.)/maize (Zea mays L.) + cowpea (Vigna sinensis L.), black oat/maize, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crop systems; Fungistasis; No-tillage; Plant diseases. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000100069 |
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| Registros recuperados: 11 | |
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